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Literature summary for 4.2.3.186 extracted from

  • Mafu, S.; Potter, K.C.; Hillwig, M.L.; Schulte, S.; Criswell, J.; Peters, R.J.
    Efficient heterocyclisation by (di)terpene synthases (2015), Chem. Commun. (Camb.), 51, 13485-13487 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.186

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of enzyme mutant H212A in Escherichia coli Fusarium fujikuroi
recombinant expression of enzyme mutant H302A in Escherichia coli Physcomitrium patens

Protein Variants

Protein Variants Comment Organism
H212A the mutant shows altered substrate specificity compared to wild-type, the single histidine to alanine mutation converts FfCPS/KS into a bifunctional ent-13-epi-manoyl oxide synthase Fusarium fujikuroi
H302A the mutant shows altered substrate specificity compared to wild-type, the single histidine to alanine mutation converts PpCPS/KS into a bifunctional ent-13-epi-manoyl oxide synthase Physcomitrium patens

Organism

Organism UniProt Comment Textmining
Fusarium fujikuroi
-
 i.e. Gibberella fujikuroi
-
Fusarium fujikuroi CBS 195.34 / IMI 58289 / NRRL A-6831
-
 i.e. Gibberella fujikuroi
-
Physcomitrium patens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme mutant H212A from Escherichia coli Fusarium fujikuroi
recombinant enzyme mutant H302A from Escherichia coli Physcomitrium patens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8alpha-hydroxy-copalyl diphosphate produced by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A Physcomitrium patens ent-13-epi-manoyl oxide + diphosphate
-
 ?
8alpha-hydroxy-copalyl diphosphate produced by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A Fusarium fujikuroi ent-13-epi-manoyl oxide + diphosphate
-
 ?
8alpha-hydroxy-copalyl diphosphate produced by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A Fusarium fujikuroi CBS 195.34 / IMI 58289 / NRRL A-6831 ent-13-epi-manoyl oxide + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
FfCPS/KS
-
 Fusarium fujikuroi

General Information

General Information Comment Organism
additional information the production of manoyl oxide proceeds from a substrate wherein oxygen is already present in the form of a hydroxyl group added during bicyclization of the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP) to 8alpha-hydroxy-copalyl diphosphate catalysed by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A, EC 5.5.1.13. 8alpha-Hydroxy-copalyl diphosphate is then converted to ent-13-epi-manoyl oxide by the modified ent-kaurene synthase function of enzyme FfCPS/KS mutant H212A Fusarium fujikuroi
additional information the production of manoyl oxide proceeds from a substrate wherein oxygen is already present in the form of a hydroxyl group added during bicyclization of the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP) to 8alpha-hydroxy-copalyl diphosphate catalysed by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A, EC 5.5.1.13. 8alpha-Hydroxy-copalyl diphosphate is then converted to ent-13-epi-manoyl oxide by the modified ent-kaurene synthase function of enzyme PpCPS/KS mutant H302A Physcomitrium patens